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Instamatic

Instamatic is a Python program that is being developed with the aim to automate the collection of electron diffraction data. At the core is a Python library for transmission electron microscope experimental control with bindings for the JEOL/FEI microscopes and interfaces to the ASI/TVIPS/Gatan cameras. Routines have been implemented for collecting serial electron diffraction (serialED), continuous rotation electron diffraction (cRED, aka 3D-ED / microED), and stepwise rotation electron diffraction (RED) data. For streaming cameras, instamatic includes a live-view GUI.

Instamatic is distributed via pypi and https://github.com/instamatic-dev/instamatic/releases. However, the most up-to-date version of the code (including bugs!) is available from this repository.

Electron microscopes supported:

  • JEOL microscopes with the TEMCOM library
  • FEI microscopes via the scripting interface

Cameras supported:

  • ASI Timepix
  • ASI CheeTah through serval-toolkit library
  • TVIPS cameras through EMMENU4 API
  • Quantum Detectors MerlinEM
  • (Gatan cameras through DM plugin [1])

Instamatic has been developed on a JEOL-2100 with a Timepix camera, and a JEOL-1400 and JEOL-3200 with TVIPS cameras (XF416/F416).

See instamatic-dev/instamatic-tecnai-server for a TEM interface to control a FEI Tecnai-TEM on Windows XP/Python 3.4 via instamatic.

[1]: Support for Gatan cameras is somewhat underdeveloped. As an alternative, a DigitalMicrograph script for collecting cRED data on a OneView camera (or any other Gatan camera) can be found here.

Installation

If you use conda, create a new environment:

conda create -n instamatic python=3.11
conda activate instamatic

Install using pip, works with python versions 3.7 or newer:

pip install instamatic

OS requirement

The package requires Windows 7 or higher. It has been mainly developed and tested under windows 7 and higher.

Package dependencies

Check pypoject.toml for the full dependency list and versions.

Documentation

See the documentation for how to set up and use Instamatic.

Reference

If you found Instamatic useful, please consider citing it or one of the references below.

Each software release is archived on Zenodo, which provides a DOI for the project and each release. The project DOI 10.5281/zenodo.1090388 will always resolve to the latest archive, which contains all the information needed to cite the release.

Alternatively, some of the methods implemented in Instamatic are described in:

Source Code Structure

  • demos/ - Jupyter demo notebooks
  • docs/ - Documentation
  • src/ - Source code for instamatic
  • src/instamatic/
  • TEMController/ - Microscope interaction code
  • calibrate/ - Tools for calibration
  • camera/ - Camera interaction code
  • config/ - Configuration management
  • experiments/ - Specific data collection routines
  • formats/ - Image formats and other IO
  • gui/ - GUI code
  • neural_network/ - Crystal quality prediction
  • processing/ - Data processing tools
  • server/ - Manages interprocess/network communication
  • utils/ - Helpful utilities
  • acquire_at_items.py - Stage movement/data acquisition engine
  • admin.py - Check for administrator
  • banner.py - Appropriately annoying thank you message
  • browser.py - Montage browsing class
  • exceptions.py - Internal exceptions
  • goniotool.py - Goniotool (JEOL) interaction code
  • gridmontage.py - Grid montage data collection code
  • image_utils.py - Image transformation routines
  • imreg.py - Image registration (cross correlation)
  • io.py - Some io-related scripts
  • main.py - Main entry point
  • montage.py - Image stitching
  • navigation.py - Optimize navigation paths
  • tools.py - Collection of functions used throughout the code
  • scripts/ - Helpful scripts
  • pyproject.toml - Dependency/build system declaration